DETECTION OF CHLOROPHYLLS c1, c2 AND c3 IN PIGMENT EXTRACTS OF PRYMNESIUM PARWM (PRYMNESIOPHYCEAE)1

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Abstract
Three chlorophyll c‐type pigments were separated by reversed‐phase high Performance liquid chromatography and thin‐layer chromatography from pigment extracts of the prymnesiophyte, Prymnesium parvum Carter. Based on spectral characteristics, retention times, and comparison with reference pigments isolated from the diatom Phaeodactylum tricornutum Bohlin, two of these pigments were identijied as chlorophyll c1 and c2. The other pigment was identified by its absorption spectrum and thin‐layer chromatography retention times as the newly described chlorophyll c3. However, in other prymnesiophytes so far examined, chlorophyll c1 and chlorophyll c3 were present with no chlorophyll cl. The discovery of chlorophyll c3 with chlorophyll c1 and chlorophyll c3 in Prymnesium parvum therefore represents the first report of this combination of pigments in prymnesiophytes.