PRELIMINARY OBSERVATIONS ON THE ANTIDIURETIC SUBSTANCE RECOVERED FROM THE URINES OF PATIENTS WITH CIRRHOSIS OF THE LIVER

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Abstract
Urines from patients with cirrhosis of the ltver in various stages of the disease were collected and pooled. The urine was dialyzed in 200 ml. aliquots against dist. water for 5 days, and then concd, by suspension before a fan. The concentrates were adsorbed on a Permutit column which was eluted with dist. water followed by 5% NaCl in 1 [image] acetic acid. The eluates were tested for antidiuretic activity by injn. into hy-drated rats following the method of Burn. Negligible amts. of antidiuretic activity were found in the water eluates. Large amts. of antidiuretic substance were found in the NaCl-acetic acid eluates at pH ranging from 5.2 to 3.9. The active eluates retained their potency during more than 4 months of storage in the refrigerator. The injn. of 2.5-10% solns. of NaCl was shown to have less antidiuretic effect than was found in the active eluates. The studies establish the presence of a dialyzable antidiuretic substance in urine whose effect when injd. into rats is similar to that of commercial Pitressin.