Improved Yields of Factor VIII from Heparinized Plasma

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Abstract
The factor VIII procoagulant activity in [human] plasma, cryoprecipitate and their polyethylene glycol (PEG) precipitates was markedly increased if blood is collected into heparin rather than into citrate phosphate dextrose (CPD). There is 34% increase in the initial level of the factor VIII activity in the heparinized plasma with 78% of this initial activity (184 U[units]) recovered in the cryoprecipitate. The stability of the factor VIII activity was improved as well. After 24 h of incubation at 22.degree. C, 99% of the initial activity was retained in heparinized plasma, whereas only 64% remained in CPD plasma. The cryoprecipitate prepared from heparinized plasma was equally stable after 24 h. The PEG concentrate prepared from the cryoprecipitate of heparinized plasma was increased to 128 U, compared to only 54 U from CPD plasma. Relative recoveries were 531 U/l for heparinized plasma versus 215 U/l for CPD plasma. This represented a 145% increase in yield.