Expression of cytochrome P‐450 3A in HT29‐MTX cells and Caco‐2 clone TC7
- 5 December 1994
- journal article
- Published by Wiley in FEBS Letters
- Vol. 355 (3) , 247-250
- https://doi.org/10.1016/0014-5793(94)01199-0
Abstract
HT-29 sublines and Caco-2 clones were analyzed for the expression of cytochrome P-450 3A. The enzyme was found to be expressed in differentiated HT-29 cells selected by resistance to methotrexate and in one of seven Caco-2 clones, TC7. Its expression parallels the differentiation process, with highest levels being observed at late confluency. P-450 3A mRNA and protein patterns, as well as subcellular distribution, are intermediate between those observed in human adult intestine and fetal liver.Keywords
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