IMPROVED METHOD FOR REPEATED PLASMAPHERESIS IN RAT

  • 1 January 1976
    • journal article
    • research article
    • Vol. 26  (1) , 93-95
Abstract
The rat was anesthetized with ketamine hydrochloride, and venipuncture of the iliac vein was performed with a 21 gauge butterfly infusion set with 12" of flexible tubing. Six ml of blood were drawn into a heparinized syringe and centrifuged. After the plasma was separated, the red blood cells were reinfused through the phlebotomy apparatus. This procedure was performed on each rat weekly for 10 wk without apparent harm to the animals. There were no changes in total serum protein and albumin, and body weights in most animals remained constant. This method should prove valuable in studies in which long-term phasmapheresis is indicated.

This publication has 6 references indexed in Scilit: