A Laboratory Antifeedant Simulation Bioassay for Phytophagous Insects12

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Abstract
A comparative bioassay was developed to evaluate the occurrence of strict antifeeding action of experimental compounds that deter feeding by phytophagous insects. The bioassay is based on starvation-induced physiological differences between cohorts of lepidopterous larvae equivalently stressed by chemical and physical means. The responses caused by guazatine triacetate were not significantly different from starvation when duration of feeding stage, larval weight change, pupal weights, and duration of pupal period were considered in 2 trials. Chemical effects, however, were significantly more severe in delaying development and seemed to cause early death after extended exposure of the larvae, particularly at high rates. Therefore, guazatine triacetate is not a strict antifeedant and should be classified as a feeding deterrent with strong antifeedant properties.