An Adenoviral Platform for Selective Self‐Assembly and Targeted Delivery of Nanoparticles
- 4 February 2008
- Vol. 4 (2) , 262-269
- https://doi.org/10.1002/smll.200700403
Abstract
Metallic nanoparticles (NPs) can be used for the diagnosis, imaging, and therapy of tumors and cardiovascular disease. However, targeted delivery of NPs to specific cells remains a major limitation for clinical realization of these potential treatment options. Herein, a novel strategy for the specific coupling of NPs to a targeted adenoviral (Ad) platform to deliver NPs to specific cells is defined. Genetic manipulation of the gene‐therapy vector is combined with a specific chemical coupling strategy. In particular, a high‐affinity interaction between a sequence of six‐histidine amino acid residues genetically incorporated into Ad capsid proteins and nickel(II) nitrilotriacetic acid on the surface of gold NPs is employed. The selective self‐assembly of gold NPs and Ad vectors into multifunctional platforms does not negatively affect the targeting of Ad to specific cells. This opens the possibility of using Ad vectors for targeted NP delivery, thereby providing a new type of combinatorial approach for the treatment of diseases that involves both nanotechnology and gene therapy.Keywords
This publication has 32 references indexed in Scilit:
- Adenovirus Tumor Targeting and Hepatic Untargeting by a Coxsackie/Adenovirus Receptor Ectodomain Anti–Carcinoembryonic Antigen Bispecific AdapterCancer Research, 2007
- Visualization of α-Helices in a 6-Ångstrom Resolution Cryoelectron Microscopy Structure of Adenovirus Allows Refinement of Capsid Protein AssignmentsJournal of Virology, 2006
- Cryoelectron Microscopy of Protein IX-Modified Adenoviruses Suggests a New Position for the C Terminus of Protein IXJournal of Virology, 2006
- Covalently Linked Au Nanoparticles to a Viral Vector: Potential for Combined Photothermal and Gene Cancer TherapyNano Letters, 2006
- MR molecular imaging and fluorescence microscopy for identification of activated tumor endothelium using a bimodal lipidic nanoparticleThe FASEB Journal, 2005
- Rapid Construction of Capsid-Modified Adenoviral Vectors Through Bacteriophage λ Red RecombinationHuman Gene Therapy, 2004
- Ensemble and single particle photophysical properties (two‐photon excitation, anisotropy, FRET, lifetime, spectral conversion) of commercial quantum dots in solution and in live cellsMicroscopy Research and Technique, 2004
- Fiber-mosaic adenovirus as a novel approach to design genetically modified adenoviral vectorsVirus Research, 2004
- In vivo cancer targeting and imaging with semiconductor quantum dotsNature Biotechnology, 2004
- Cellular Trajectories of Peptide-Modified Gold Particle Complexes: Comparison of Nuclear Localization Signals and Peptide Transduction DomainsBioconjugate Chemistry, 2004