Turnover of Hepatic Catalase Modified by Aminotriazole

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Abstract
1) The turnover of inactivated catalase [EC 1.11.1.6] was studied by injection of radioactive 3-amino-1,2,4-triazole(AT). The livers of rats injected with 14C-AT were fractionated into peroxisomal and soluble fractions and the disappearance of the radioactivity from protein precipitated with anti-catalase antibody was determined. The half-life of the radioactivity in catalase protein was about 2 days for peroxisomes and less than a day for cytosol. 2) The immunoprecipitation curves of catalase and AT-catalase obtained with anti-catalase antiserum demonstrated that catalase and AT-catalase were identical in their antigenic properties and formed the same amount of immunoprecipitate with anti-catalase antiserum. 3) Changes in the amounts of immunoprecipitates in peroxisomes and cytosol were determined after treatment with AT. The amount of immunoprecipitates in the peroxisomes remained unchanged after inhibition by AT, which indicates that the peroxisomal catalase behaves in the degradation process in a way which is essentially unaltered by inactivation. On the other hand, the immunoprecipitate in cytosol decreased on AT treatment, suggesting that the degradation rate of cytosol catalase is accelerated by the modification.

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