Sequences of initiator and elongator methionine tRNAs in bean mitochondria

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Abstract
Two bean mitochondria methionine transfer RNAs, purified by RPC-5 chromatography and two-dimensional gel electrophoresis, have been sequenced usingin vitro post-labeling techniques. One of these tRNAsMet has been identified by formylation using anE. coli enzyme as the mitochondrial tRNAFMet. It displays strong structural homologies with prokaryotic and chloroplast tRNAFMet sequences (70.1–83.1%) and with putative initiator tRNAmMet genes described for wheat, maize andOenothera mitochondrial genomes (88.3–89.6%). The other tRNAMet, which is the mitochondrial elongator tRNAFMet, shows a high degree of sequence homology (93.3–96%& with chloroplast tRNAmMet, but a weak homology (40.7%) with a sequenced maize mitochondrial putative elongator tRNAmMet gene. Bean mitochondrial tRNAFMet and tRNAmMet were hybridized to Southern blots of the mitochondrial genomes of wheat and maize, whose maps have been recently published (15, 22), in order to locate the position of their genes.