Enzymic Formation of Di-D-Fructose 1,2'; 2,1' Dianhydride from Inulobiose by Aspergillus fumigatus

Abstract
The enzyme of Aspergillus fumigatus which produces di-D-fructose 1,2'; 2,1' dian-hydride (difructose anhydride I) (Tanaka et al., 1979) was investigated for its mode of action. Fractionation and purification of the enzyme showed that it produced difructose anhydride I not from inulin but from inulobiose by the reverse reaction of the partial hydrolysis of difructose anhydride I.

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