Crystals of the chemically synthesized acceptor stem of tRNAAlafromEscherichia colidiffracting to high resolution
- 1 July 1996
- journal article
- research article
- Published by International Union of Crystallography (IUCr) in Acta Crystallographica Section D-Biological Crystallography
- Vol. 52 (4) , 871-873
- https://doi.org/10.1107/s0907444996000455
Abstract
The acceptor stem of tRNA(Ala) from E. coli has been chemically synthesized and crystallized. This duplex contains a G.U base pair in position 3-70, which is the main identity element for alanyl-tRNA synthetase from E. coli. The crystals are stable in the X-ray beam for a long period of time and diffract to 1.7 A resolution. The monoclinic crystals reveal a C2 space group with a = 35.0, b = 47.5, c = 26.2 A, beta = 102.3 degrees and one acceptor stem per asymmetric unit.Keywords
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