Aryldiazomethanes for Universal Labeling of Nucleic Acids and Analysis on DNA Chips
- 31 October 2003
- journal article
- Published by American Chemical Society (ACS) in Bioconjugate Chemistry
- Vol. 14 (6) , 1298-1306
- https://doi.org/10.1021/bc0341371
Abstract
DNA and RNA labeling and detection are key steps in nucleic acid-based technologies, used in medical research and molecular diagnostics. We report here the synthesis, reactivity, and potential of a new type of labeling molecule, m-(N-Biotinoylamino)phenylmethyldiazomethane (m-BioPMDAM), that reacts selectively and efficiently with phosphates in nucleotide monomers, oligonucleotides, DNA, and RNA. This molecule contains a biotin as detectable unit and a diazomethyl function as reactive moiety. We demonstrate that this label fulfills the requirements of stability, solubility, reactivity, and selectivity for hybridization-based analysis and especially for detection on high-density DNA chips.Keywords
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