Techniques for Mammalian Cell Tissue Culture
Open Access
- 1 April 2006
- journal article
- appendix
- Published by Wiley in Current Protocols in Molecular Biology
- Vol. 74 (1) , A.3F.1-A.3F.18
- https://doi.org/10.1002/0471142727.mba03fs74
Abstract
This appendix opens with detailed discussions on the latest principles of sterile technique and preparation of culture media. Step‐by‐step protocols describe trypsinizing and subculturing monolayer cultures, passaging suspension cultures, freezing and thawing cells, counting cells using a hemacytometer, and preparing cells for transport.Keywords
This publication has 1 reference indexed in Scilit:
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