Aging in vitro and large-scale interferon production by 15 new strains of human diploid fibroblasts

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Abstract
To develop resources for large-scale production of human fibroblast interferon, 15 new strains of human diploid foreskin fibroblasts were isolated, cryopreserved and characterized. Their life spans in vitro ranged from 52-72 population doublings. Selection of cell strains for mass interferon production was based on the number of population doublings during which consistently high yields of interferon were obtained after "superinduction" in roller bottles. Aging in vitro leads to significant decline in amounts of interferon produced. Susceptibility to interferon remains largely unaffected by in vitro senescence. Karyotypic analysis indicated that the best interferon-producing strain, MLD (over 60,000 reference units/ml), has a translocation between chromosomes 5 and 15.