Androgenesis in vitro in anther cultures of chlorophyll mutants ofNicotiana tabacum
- 1 July 1973
- journal article
- Published by Institute of Experimental Botany in Biologia plantarum
- Vol. 15 (4) , 286-289
- https://doi.org/10.1007/bf02922711
Abstract
The course of androgenesisin vitro was investigated with anther cultures of two chlorophyll mutants of Nicotiana tabacum. A different sensitivity to the hormonal composition of the medium was revealed between the cultures White Seedling and Sulfur; the stimulatory effect of kinetin on the frequency of androgenesis was observed only in White Seedling cultures. In addition to green plants, “aurea” (mutation Sulfur) or “albino” (mutation White Seedling) phenotypes also differentiated in both cultures. The possible causes of variability in the participation of green and mutant forms are discussed. Průběh androgenese in vitro byl sledovän v prašníkových kulturäch dvou chlorofylových mutantNicotiana tabacum L. Byla zjištěna rozdílná citlivost kultur White Seedling a Sulfur k hormonálnímu složení media; stimulační efekt kinetinu na frekvenci androgenese byl pozorován pouze u kultur White Seedling. V obou kulturách se kromě zelených rostlin diferencovaly též rostliny fenotypu „aurea” (mutace Sulfur) či „albino“ (mutace White Seedling). Možné příčiny variability v zastoupeí zelených a mutantních forem jsou diskutovány.Keywords
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