Simultaneous production of three phenazine pigments by Pseudomonas aeruginosa Mac 436

Abstract
Pseudomonas aeruginosa Mac 436 was found to produce simultaneously three phenazine pigments identified as pyocyanine, phenazine-1-carboxylic acid, and oxychlororaphine. Production of these pigments on various media indicated a wide variation in yields depending on the composition of the media, but satisfactory yields of all three pigments were obtained. A scheme was developed for separation and assay of the pigments from the culture liquor. Details of production, isolation, assay, and identification are given.

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