Initial and Persisting Staining power of Solutions of Iron-Hematoxylin Lake
- 1 January 1949
- journal article
- research article
- Published by Taylor & Francis in Stain Technology
- Vol. 24 (3) , 157-163
- https://doi.org/10.3109/10520294909139598
Abstract
The stain is made up as follows: in 50 ml. water dissolve, in order, 1 g. ferric ammonium sulfate [FeNH4 (SO4)2. 12H2O], 0.8 ml. H2SO4, 50 ml. 95% ethyl alcohol, 0.5 g. hematoxylin. Filter the soln. to remove the insoluble, white crust of the Fe ammonium sulfate. The soln. stains well 10 min. after it has been made. Peak performance is attained within 5 hrs., and is maintained for 4-8 wks. Staining time is 3-30 min. Excess stain can be rinsed off the slide and section by immersion in water, after which destaining, if necessary, can be accomplished with a soln. of 50 ml. water, 50 ml. 95% ethyl alcohol, 0.18 ml. H2SO4. The slides may or may not be placed next in a neutralizing soln. of 50 ml. water, 50 ml. 95% ethyl alcohol, 0.5 g. NaHCO3.Keywords
This publication has 2 references indexed in Scilit:
- Studies on Hematoxylin StainsStain Technology, 1943
- The Use of Buffered Solutions in Staining: Theory and PracticeStain Technology, 1937