Rapid purification of protein phosphatase 2A from mouse brain by microcystin‐affinity chromatography
- 11 February 1991
- journal article
- Published by Wiley in FEBS Letters
- Vol. 279 (1) , 115-118
- https://doi.org/10.1016/0014-5793(91)80264-4
Abstract
Microcystin LR. which is a monocyclic heptapeptide containing two L-amino acids, and leucine and arginine, is a new inhibitor of protein phosphatases 1 and 2A. Microcystin LR-affinity chromatography was used to purify protein phosphatase 2A as a holoenzyme. Five mg of microcystin LR were immobilized to ECH Sepharose 4B by the carbodiimide coupling reaction. Following DEAE-cellulose column chromatography, microcystin-affinity chromatography, as the second step in the procedure, resulted in purification of protein phosphatase 2A in a pure form. The enzyme isolated from mouse brain consisted of two regulatory subunits of 67 kDa and 58 kDa and a catalytic subunit of 41 kDa. Microcystin-affinity chromatography is useful for isolation of protein phosphatase 2A.Keywords
This publication has 13 references indexed in Scilit:
- Inhibition of protein phosphatases by microcystis and nodularin associated with hepatotoxicityZeitschrift für Krebsforschung und Klinische Onkologie, 1990
- Structure-activity relationship within a series of okadaic acid derivativesCarcinogenesis: Integrative Cancer Research, 1990
- Distinct, essential roles of type 1 and 2A protein phosphatases in the control of the fission yeast cell division cycleCell, 1990
- Photoaffinity labeling of protein phosphatase 2A, the receptor for a tumor promoter okadaic acid, by [27-3H]methyl 7- -(4-azidobenzoyl)okadaateBiochemical and Biophysical Research Communications, 1990
- Association of protein phosphatase 2A with polyoma virus medium tumor antigen.Proceedings of the National Academy of Sciences, 1990
- Regulation of collagenase gene expression by okadaic acid, an inhibitor of protein phosphatases.Cell Regulation, 1990
- THE STRUCTURE AND REGULATION OF PROTEIN PHOSPHATASESAnnual Review of Biochemistry, 1989
- Apparent “activation” of protein kinases by okadaic acid class tumor promotersBiochemical and Biophysical Research Communications, 1989
- Diarrhetic Shellfish Toxin, Dinophysistoxin‐1, Is a Potent Tumor Promoter on Mouse SkinJapanese Journal of Cancer Research, 1988
- Okadaic acid: an additional non-phorbol-12-tetradecanoate-13-acetate-type tumor promoter.Proceedings of the National Academy of Sciences, 1988