New Method for Extraction of Ribonucleic Acid and Polyribosomes from Schizosaccharomyces pombe
- 1 September 1971
- journal article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 107 (3) , 659-63
- https://doi.org/10.1128/jb.107.3.659-663.1971
Abstract
A new method for extracting ribosomal ribonucleic acid (RNA) and polyribosomes from Schizosaccharomyces pombe , a fission yeast, has been developed. Cells grown in the presence of 2-deoxyglucose were found to be more sensitive to the action of Glusulase; a short period of treatment (10 to 20 min) at 0 C was sufficient to convert the cells to osmotically sensitive structures. From these, ribosomal RNA and polyribosomes were readily prepared.Keywords
This publication has 16 references indexed in Scilit:
- Secretion of cell-wall glycoproteins by yeast protoplasts. Effect of 2-deoxy-d-glucose and cycloheximideBiochemical Journal, 1970
- Sedimentation characteristics of RNA from isolated yeast nucleiExperimental Cell Research, 1970
- Chapter 7 Physiological and Cytological Methods for Schizosaccharomyces pombePublished by Elsevier ,1970
- Chapter 8 Genetical Methods for Schizosaccharomyces pombePublished by Elsevier ,1970
- The synthesis and processing of ribosomal RNA precursor molecules in yeastBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1969
- Ribonucleic acids from animal cells.1968
- Ribosomal Precursor RNA in Saccharomyces carlsbergensisEuropean Journal of Biochemistry, 1967
- Macromolecule Synthesis in Yeast SpheroplastsJournal of Bacteriology, 1967
- Ribonucleic acid synthesis in yeast. The effect of cycloheximide on the synthesis of ribonucleic acid in Saccharomyces carlsbergensisBiochemical Journal, 1966
- RNA metabolism in the HeLa cell nucleusJournal of Molecular Biology, 1966