Purification of DNA-dependent RNA polymerase fromStreptomyces granaticolor
- 1 September 1983
- journal article
- research article
- Published by Springer Nature in Folia Microbiologica
- Vol. 28 (5) , 435-438
- https://doi.org/10.1007/bf02879496
Abstract
RNA nucleotidyltransferase (EC 2.7.7.6) ofStreptomyces granaticolor was purified by precipitation with polymin P and ammonium sulphate, affinity chromatography on DNA-cellulose and gell filtration on Biogel A 1.5 m. SDS-polyacrylamide gel electrophoresis revealed 8 protein bands of molar mass ranging from 37 to 130 kg/mol. Proteins of molar mass of 130 and 120 kg/mol were identified to be β and β subunits, respectively. The role of other subunits of the enzyme is discussed.Keywords
This publication has 8 references indexed in Scilit:
- Inhibitory effects of 3′-deoxycytidine 5′-triphosphate and 3′-deoxyuridine 5′-triphosphate on DNA-dependent RNA polymerases I and II purified from Dictyostelium discoideum cellsNucleic Acids Research, 1981
- Differential staining of the subunits of DNA‐dependent RNA polymeraseFEBS Letters, 1980
- Purification of RNA polymerase from actinomycin producing and nonproducing cells of Streptomyces antibioticusArchives of Biochemistry and Biophysics, 1979
- Sensitivity of transcription by purified Streptomyces antibioticus RNA polymerase to actinomycinBiochemical and Biophysical Research Communications, 1978
- Effect of rifampicin on invitro RNA synthesis of streptomycesmediterraneiBiochemical and Biophysical Research Communications, 1976
- RNA synthesis in Streptomyces antibioticus: in vitro effects of actinomycin and transcriptional inhibitors from 48-h cellsBiochemistry, 1976
- [11] DNA-cellulose chromatographyPublished by Elsevier ,1971
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970