Heterologous expression of cDNAs encoding barley (Hordeum vulgare) (1 → 3)‐β‐glucanase isoenzyme GV

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Abstract
Two cDNAs have been isolated from libraries generated from poly(A)+RNA of young barley roots and leaves, using a cDNA encoding barley (1 → 3)‐β‐glucanase isoenzyme GII as a probe. Nucleotide sequence analyses and ribonuclease protection assays show that the two cDNAs differ only in the length of their 3′‐untranslated regions; the corresponding mRNAs are likely to originate from a single gene by tissue‐specific processing at separate polyadenylation sites. When the coding region of the cDNA is expressed in E. coli, the resultant protein catalyses the hydrolysis of (1 → 3)‐β‐glucan with an action pattern characteristic of a (1 → 3)‐β‐glucan endohydrolase (EC 3.2.1.39). The enzyme has been designated isoenzyme GV of the barley (1 → 3)‐β‐glucanase family.