Steady state growth of avena coleoptile sections in high auxin concentrations

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Abstract
The extension growth of Avena sativa var. Siegeshafter coleoptile sections with time was measured in supraoptimal (10-5M and higher) concentrations of the auxin indole-3-acetic acid. Coleoptiles were obtained from seedlings grown over distilled water or in vermiculite of North American or of South African origin. Progress curves were characterized by a long period of. steady growth preceded in all cases, except for seedlings grown in North American vermiculite, by an initial transient period of more rapid growth. If coleoptile sections are preincubated for approximately 2 hours in basal medium containing auxin of the desired final concentration, reproducible steady state growth rates can be obtained in supraoptimal auxin concentrations. The steady state rates are inversely proportional to auxin concentration as expected on the basis of the 2 point attachment concept.

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