Expression of New Antigens by HL-A Variants of Cultured Lymphoid Cells

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Abstract
Antisera were raised in rabbits to a number of spontaneously occurring and mutagen-induced HL-A2 loss variants of the cultured lymphoid line 8866. The antisera to the variant sublines were absorbed with 8866 and with one or both of the spontaneously occurring variant sublines, and the absorbed antisera were tested to determine whether they retained reactivity for the variant against which the antiserum was raised. A positive result was taken as evidence that the variant expressed a new antigen. By this criterion, neither spontaneously occurring variant appeared to express a new antigen. However, at least two of four mutagen-induced variants appeared to do so. Antiserum against 8866 absorbed with either of the spontaneously occurring HL-A2 loss variants appeared to be a specific HL-A2 reagent, supporting the idea that antigen loss in these variants is limited to the HL-A2 allele.

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