Temperature-sensitive Mutants of the Streptomyces Plasmid pIJ702
- 1 June 1985
- journal article
- research article
- Published by Microbiology Society in Microbiology
- Vol. 131 (6) , 1299-1303
- https://doi.org/10.1099/00221287-131-6-1299
Abstract
DNA from the Streptomyces plasmid pIJ702 was mutagenized in vitro using hydroxylamine and transformed into S. lividans. One plasmid with temperature-sensitive replication (pMT660) and 1 plasmid with a temperature-sensitive tyrosinase (pMT661) were isolated. Plasmid pMT661 contains a novel PstI restriction endonuclease site within the tyrosinase gene.This publication has 1 reference indexed in Scilit: