Effect of pH, phosphate, and ADP on relaxation of myocardium after photolysis of diazo 2

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Abstract
The aim of this study was to examine the effect of the metabolites H+, ADP, and Pion the rate of cardiac relaxation. We used guinea pig right ventricular trabeculae that had been chemically skinned, allowing the myofilaments to be studied in isolation. Laser-flash photolysis of the caged Ca2+chelator diazo 2, causing a rapid fall in intracellular Ca2+, enabled investigation of relaxation independently of the rate of Ca2+diffusion. On the photolysis of diazo 2, the trabeculae relaxed biphasically with exponential rate constants ( k1and k2) of 10.07 and 4.23 s−1, respectively, at 12°C and 18.35 and 2.52 s−1, respectively, at a nominal 20°C. Increasing the concentration of both protons (pH 7.2–6.8) and MgADP (0.5–3.4 mM) slowed the two phases of the relaxation transients. Raising the concentration of Pifrom the control level of 1.36 mM to 15.2 mM increased the rate of both phases, with relaxation becoming monoexponential at 19.4 mM Pi(with a k of 20.31 s−1at 12°C). Cardiac muscle was compared with skeletal muscle under identical conditions; in cardiac muscle 19.4 mM Piincreased the rate of relaxation, whereas in skeletal muscle this concentration of Pislowed relaxation. We conclude that the mechanism of relaxation differs between cardiac and skeletal muscle. This study is a direct demonstration of the effects of ATP metabolites on cardiac myofilament processes during relaxation.