A New Redox Cofactor in Eukaryotic Enzymes: 6-Hydroxydopa at the Active Site of Bovine Serum Amine Oxidase
- 25 May 1990
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 248 (4958) , 981-987
- https://doi.org/10.1126/science.2111581
Abstract
An active site, cofactor-containing peptide has been obtained in high yield from bovine serum amine oxidase. Sequencing of this pentapeptide indicates: Leu-Asn-X-Asp-Tyr. Analysis of the peptide by mass spectrometry, ultraviolet-visible spectroscopy, and proton nuclear magnetic resonance leads to the identification of X as 6-hydroxydopa. This result indicates that, contrary to previous proposals, pyrroloquinoline quinone is not the active site cofactor in mammalian copper amine oxidases. Although 6-hydroxydopa has been implicated in neurotoxicity, the data presented suggest that this compound has a functional role at an enzyme active site.This publication has 32 references indexed in Scilit:
- Reductive trapping of substrate to methylamine oxidase from Arthrobacter P1FEBS Letters, 1990
- Primary structure of a pyrroloquinoline quinone (PQQ) containing peptide isolated from porcine kidney diamine oxidaseBiochemical and Biophysical Research Communications, 1989
- Evidence for PQQ as cofactor in 3,4‐dihydroxyphenylalanine (dopa) decarboxylase of pig kidneyFEBS Letters, 1988
- L‐tyrosine is the precursor of PQQ biosynthesis in Hyphomicrobium XFEBS Letters, 1988
- Pyrroloquinoline quinone (PQQ) is the organic cofactor in soybean lipoxygenase‐1FEBS Letters, 1988
- The interaction of aminogroups with pyrroloquinoline quinone as detected by the reduction of nitroblue tetrazoliumBiochemical and Biophysical Research Communications, 1988
- Dopamine β‐hydroxylase from bovine adrenal medulla contains covalently‐bound pyrroloquinoline quinoneFEBS Letters, 1988
- Phenylhydrazine as probe for cofactor identification in amine oxidoreductases Evidence for PQQ as the cofactor in methylamine dehydrogenaseFEBS Letters, 1987
- Bovine serum amine oxidase: a mammalian enzyme having covalently bound PQQ as prosthetic groupFEBS Letters, 1984
- The molecular, mechanistic and immunological properties of amine oxidasesMolecular and Cellular Biochemistry, 1976