A NOVEL METHOD TO IMPROVE IMMUNOFLUORESCENCE IN HISTOLOGICAL SPECIMENS
- 1 January 1980
- journal article
- research article
- Vol. 21 (1) , 134-138
Abstract
A new approach to immunofluorescent labeling of sectioned tissue is described. Small tissue fragments which were fixed and rendered permeable are labeled with immune reagents by either the direct or indirect method, prior to embedding in glycol methacrylate (2-hydroxyethyl methacrylate) at 4.degree.. In this way, the well-known superiority of plastic embedding over paraffin embedding or cryo-sections can be used to compare cellular structure in tissues with that seen in cultured cells. The method was applied to tissues from the chick embryo or young hatched chicken.This publication has 8 references indexed in Scilit:
- A general method for permeabilizing monolayer and suspension cultured animal cellsExperimental Cell Research, 1979
- The pH dependence of borohydride as an aldehyde reductantJournal of Molecular Histology, 1979
- Ultrastructure of chick tendon fibroblast with special reference to secretory mechanismCells Tissues Organs, 1979
- Primary avian tendon cells in culture. An improved system for understanding malignant transformation.The Journal of cell biology, 1978
- Cytoplasmic microtubular images in glutaraldehyde-fixed tissue culture cells by electron microscopy and by immunofluorescence microscopyProceedings of the National Academy of Sciences, 1978
- An efficient method to produce specific anti-actinHistochemistry and Cell Biology, 1978
- Science and Art in Preparing Tissues Embedded in Plastic for Light Microscopy, with Special Reference to Glycol Methacrylate, Glass Knives and Simple StainsStain Technology, 1976
- A PARAFFIN EMBEDDING TECHNIQUE FOR STUDIES EMPLOYING IMMUNOFLUORESCENCEJournal of Histochemistry & Cytochemistry, 1962