Radioimmunoassay of the Insulin Receptor: A New Probe of Receptor Structure and Function
- 9 February 1979
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 203 (4380) , 544-547
- https://doi.org/10.1126/science.83675
Abstract
A sensitive and specific radioimmunoassay for the insulin receptor has been developed employing receptor autoantibodies from the serum of a patient with insulin-resistant diabetes. The assay detects insulin binding sites at concentrations as low as 0.1 nanomolar; distinguishes between receptors originating from human placental membranes, human lymphoblastoid cells, and mouse liver membranes; and measures the receptor independently of its binding function. Down-regulation, or loss of binding after exposure to insulin, is associated with loss of immunoreactive receptor.Keywords
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