Mixed Reversed Phase/Beta Cyclo-Dextrin Packings in High Performance Liquid Chromatography: Single Mixed Support Column Versus Two Columns in Series

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Abstract
A study of mixed C18/β packings in high performance liquid chromatography was undertaken to evaluate the utility of such phases. The results show that non additive retention times are obtained when a mixed phase column is used while the retention times are additive when two columns, one packed with C18 and the other with β are coupled in a series. A plot of corrected retention times vs % β in C18 gave a non-linear result. This means that the prediction of retention times on a mixed phase column may not be easily done. As a result, in this case, it will be easier to couple two different columns of different selectivities to achieve a difficult separation.