A previously unrecognized glutamine synthetase expressed in Klebsiella pneumoniae from the glnT locus of Rhizobium leguminosarum
- 1 September 1990
- journal article
- research article
- Published by Springer Nature in Molecular Genetics and Genomics
- Vol. 223 (3) , 513-516
- https://doi.org/10.1007/bf00264461
Abstract
Using glnT DNA of Rhizobium meliloti as a hybridization probe we identified a R. leguminosarum biovar phaseoli (R. l. phaseoli) locus (glnT) expressing a glutamine synthetase activity in Klebsiella pneumoniae. A 2.2 kb DNA fragment from R. l. phaseoli was cloned to give plasmid pMW5a, which shows interspecific complementation of a K. pneumoniae glnA mutant. The cloned sequence did not show cross-hybridization to glnA or glnII, the genes coding for two glutamine synthetase isozymes of Rhizobium spp. While in previous reports on glnT of R. meliloti and Agrobacterium tumefaciens no glutamine synthetase activity was detected, we do find activity with the glnT locus of R. l. phaseoli. The glutamine synthetase (GSIII) activity expressed in a K. pneumoniae glnA strain from pMW5a shows a ratio of biosynthetic to transferase activity 103-fold higher than that observed for GSI or GSII. GSIII is similar in molecular weight and heat stability to GSI.This publication has 31 references indexed in Scilit:
- Cloning and analysis of Agrobacterium tumefaciens C58 loci involved in glutamine biosynthesis: Neither the glnA (GSI) nor the glnII (GSII) gene plays a special role in virulenceMolecular Genetics and Genomics, 1988
- Characterization and Cloning of Two Rhizobium leguminosarum Genes Coding for Glutamine Synthetase ActivitiesMicrobiology, 1986
- Apparent eukaryotic origin of glutamine synthetase II from the bacterium Bradyrhizobium japonicumNature, 1986
- Identification and characterization of three forms of glutamine synthetase unique to RhizobiaCurrent Microbiology, 1986
- Impaired nitrogen fixation and glutamine synthesis in methionine sulfoximine sensitive (MSs) mutants of Rhizobium phaseoliMolecular Genetics and Genomics, 1985
- Covalent modification of bacterial glutamine synthetase: physiological significanceMolecular Genetics and Genomics, 1984
- REGULATION OF THE NITROGEN FIXATION (nif) GENES OF Klebsiella pneumoniae AND Rhizobium meliloti: ROLE OF NITROGEN REGULATION (ntr) GENESPublished by Springer Nature ,1984
- A Broad Host Range Mobilization System for In Vivo Genetic Engineering: Transposon Mutagenesis in Gram Negative BacteriaBio/Technology, 1983
- Cloning of the glnA, ntrB and ntrC genes of Klebsiella pneumoniae and studies of their role in regulation of the nitrogen fixation (nif) gene clusterMolecular Genetics and Genomics, 1982
- Two forms of glutamine synthetase in free-living root-nodule bacteriaBiochemical and Biophysical Research Communications, 1977