Stereochemistry of phosphoryl transfer
- 26 June 1981
- journal article
- review article
- Published by The Royal Society in Philosophical Transactions of the Royal Society of London. B, Biological Sciences
- Vol. 293 (1063) , 75-92
- https://doi.org/10.1098/rstb.1981.0062
Abstract
A general method has been developed for the synthesis of chiral [ 16 O, 17 O, 18 O] phosphate monoesters of known absolute configuration. An analytic method for determining the absolute configuration of chiral phosphate esters has also been developed, which is based on the isotope effects of 17 O and 18 O at phosphorus in the 31 P nuclear magnetic resonance spectrum. These methods have shown that phosphoryl transfer catalysed by hexokinase, phosphofructokinase and pyruvate kinase occurs with inversion of configuration. This is most simply interpreted as an ‘in-line’ transfer of the phosphoryl group between substrates in the enzyme-substrate ternary complex.Keywords
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