Ultraviolet-Absorption Spectra of Dry Bacterial Spores

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Abstract
The possibility of obtaining reasonably satisfactory ultraviolet-absorption spectra of dry spores embedded in KBr has been demonstrated. Such spectra show the three peaks characteristic of calcium-dipicolinate. The dipicolinate spectra are more distinct when reference pellets containing appropriate amounts of the analogous spore coats are employed. These spectra are considered evidence that some type of calcium-dipicolinic-acid chelate accounts for at least part of the calcium and dipicolinic-acid content of the spore.