Inheritance of Altered Palmitic Acid Percentage in Two Soybean Mutants

Abstract
Palmitic acid is the major saturated fatty acid in soybean seed oil. Two mutant soybean lines with altered levels of palmitic acid in their oil were crossed and studied in F19, F21, and F31, generations and in the F2, generation of crosses to the cultivar “Century,” which was the source of the mutations. Frequency distributions for palmitic acid percentage displayed five peaks among F2, seeds from F1, plants and one, three, or five peaks among F2, seeds from individual F2, plants. Three peaks were observed in the F, generation of crosses between the mutants and “Century.” Chisquare analysis of these distributions indicated that aileles from two independent loci segregated for palmitic acid percentage and that the alleles were additive in gene action. We propose gene symbols for the mutant alleles at these loci: fap2 for the allele in C1726 that acts to lower the palmitic acid percentage and fap2 for the allele in C1727 that acts to increase the palmitic acid percentage in soybean seed oil.

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