Quantitative Analysis of Collagens and Fibronectin Expression in Human Right Ventricular Hypertrophya
- 1 June 1999
- journal article
- Published by Wiley in Annals of the New York Academy of Sciences
- Vol. 874 (1 HEART IN) , 278-285
- https://doi.org/10.1111/j.1749-6632.1999.tb09243.x
Abstract
One of the main features in human tetralogy of Fallot (TF) is right ventricular hypertrophy (RVH) due to pressure (sub-pulmonary stenosis) and volume overload (ventricular septal defect). Currently, primary correction at a young age is the treatment of choice. To unravel the role of extracellular matrix in RVH, we examined myocardial expression of collagens and fibronectin in TF patients with primary correction (TF1, age 0.7 +/- 0.2 yr.), secondary surgery (TF2, age 36.9 +/- 4.6 yr), and in age-matched control patients. Sirius red staining quantified by video imaging showed significantly increased interstitial staining for collagens in both TF1 and TF2 groups as compared to respective controls. Fibronectin was expressed in extracellular spaces, perivascular regions, and in some cardiomyocytes. Quantitative analysis of fibronectin revealed increased expression in only TF1 group as compared to respective control. Our results indicate an increased amount of myocardial extracellular matrix deposition as a sign of fibrosis during RVH in patients with TF.Keywords
This publication has 19 references indexed in Scilit:
- Collagen and ventricular remodeling after acute myocardial infarction: concepts and hypothesesBasic Research in Cardiology, 1997
- Increased collagen synthesis and decreased collagen degradation in right ventricular hypertrophy induced by pressure overloadCardiovascular Research, 1994
- Sarcoplasmic reticulum gene expression in cardiac hypertrophy and heart failure.Circulation Research, 1994
- Collagen Network of the Myocardium: Function, Structural Remodeling and Regulatory MechanismsJournal of Molecular and Cellular Cardiology, 1994
- Growth and hypertrophy of the heart: towards an understanding of cardiac specific and inducible gene expressionCardiovascular Research, 1993
- Accuracy of exercise 201Tl myocardial scintigraphy in asymptomatic young men.Circulation, 1993
- Regulation of fibrillar collagen types I and III and basement membrane type IV collagen gene expression in pressure overloaded rat myocardium.Circulation Research, 1990
- The significance of coronery reserve in clinical heart diseaseJournal of the American College of Cardiology, 1990
- Fibrillar collagen and myocardial stiffness in the intact hypertrophied rat left ventricle.Circulation Research, 1989
- A morphometrical study of myocardial disarray associated with right ventricular outflow tract obstruction.Japanese Circulation Journal, 1984