Starvation or diabetes decreases the content but not the mRNA of 6‐phosphofructo‐2‐kinase in rat liver
- 25 January 1988
- journal article
- Published by Wiley in FEBS Letters
- Vol. 227 (2) , 136-140
- https://doi.org/10.1016/0014-5793(88)80884-6
Abstract
In rat liver, the activity of 6-phosphofructo-2-kinase (PFK-2) decreases upon starvation and in diabetes. Cyclic AMP-dependent phosphorylation of the enzyme is not sufficient to account for this decrease. PFK-2 content was therefore measured by immunotitration and relative PFK-2 mRNA levels were determined by hybridization with cDNA probes. The data are compatible with a posttranscriptional mechanism of regulation that involves decreased translational efficiency of PFK-2 mRNA and(or) increased turnover of the PFK-2 protein.Keywords
This publication has 21 references indexed in Scilit:
- Complete nucleotide sequence coding for rat liver 6‐phosphofructo‐2‐kinase/fructose‐2,6‐bisphosphatase derived from a cDNA cloneFEBS Letters, 1987
- Reaction of phosphofructokinase 2/fructose 2,6-bisphosphatase with monoclonal antibodies. A proof of the bifunctionality of the enzymeEuropean Journal of Biochemistry, 1986
- Translational control of gene expression in a normal fibroblast. Characterization of a subclass of mRNAs with unusual kinetic propertiesBiochemistry, 1986
- Effects of diabetes on fructose 2,6-P2, glucose 1,6-P2 and 6-phosphofructo 2-kinase in rat liverBiochemical and Biophysical Research Communications, 1986
- False starts in translational control of gene expressionNature, 1985
- Preparation and characterization of mRNAs from rat heart muscleBiochimie, 1983
- The tissue distribution of fructose-2,6-P2 and fructose-6-P,2-kinase in rats and the effect of starvation diabetes and hypoglycemia on hepatic fructose-2,6-P2 and fructose-6-P,2-kinaseBiochemical and Biophysical Research Communications, 1982
- Purification of Mouse Immunoglobulin Heavy‐Chain Messenger RNAs from Total Myeloma Tumor RNAEuropean Journal of Biochemistry, 1980
- Isolation of biologically active ribonucleic acid from sources enriched in ribonucleaseBiochemistry, 1979
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnalytical Biochemistry, 1976