THE EXTRACTION OF NITROGENOUS MATERIALS FROM PEAR TISSUES

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Abstract
The problems investigated were: (1) a suitable method of preservation of tissues; (2) methods for obtaining aqueous extract; and (3) the N fractions found in expressed sap. Effects of preservation by desiccation at 50 and 85[degree] C, freezing, cold, non-freezing temp., and chemical reagents were studied. Dehydration at 50-55[degree] C, storage at 0[degree] C. for short periods, or freezing give results similar to fresh tissue. Injured tissue changes rapidly. Since only 10-25% of total N is soluble in the sap, to see how much of the rest could be made soluble the finely divided tissue was extracted with water containing different ions or hydrolyzed with acid or animal enzymes. The N extracts were fractionated into "protein" and "non-protein.".