Partial Purification of the Template-Active Fraction of Chromatin: A Preliminary Report
- 1 June 1974
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 71 (6) , 2193-2197
- https://doi.org/10.1073/pnas.71.6.2193
Abstract
A fraction of rat-liver chromatin that is transcriptionally active in vivo has been purified 6- to 7-fold over whole chromatin. This was accomplished by selectively shearing chromatin with DNase II followed by fractionating the released portion on the basis of its solubility properties in 2 mM MgCl(2). The resulting soluble material comprises 11% of the total chromatin DNA and is impoverished in histone and enriched in nonhistone protein. Compared with unsheared chromatin, this minor fraction exhibits marked differences in chromosomal protein species. DNA renaturation studies indicate that this fraction is composed of a specific subset of whole genomal DNA sequences. Furthermore, DNA.RNA hybridization experiments suggest that almost 60% of the nonrepetitious DNA sequences of this minor fraction could code for cellular RNA.Keywords
This publication has 17 references indexed in Scilit:
- Synthesis of Globin Ribonucleic Acid from Duck-Reticulocyte Chromatin In VitroProceedings of the National Academy of Sciences, 1973
- Preparation of highly purified ribonucleic acid polymerase. Separation from polynucleotide phosphorylase and polyphosphate kinaseBiochemistry, 1972
- The structure of chromatin as revealed by deoxyribonuclease digestion studiesBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1972
- Transcription of nonrepeated DNA during mouse and rabbit developmentDevelopmental Biology, 1972
- Fractionation of chromatin by thermal chromatographyBiochemistry, 1972
- Fractionation of Liver ChromatinProceedings of the National Academy of Sciences, 1971
- Repetitive and Non-Repetitive DNA Sequences and a Speculation on the Origins of Evolutionary NoveltyThe Quarterly Review of Biology, 1971
- Iodination of nucleic acids in vitroBiochemistry, 1971
- Studies on the nuclear 4 to 7 s RNA of the novikoff hepatomaJournal of Molecular Biology, 1969
- High resolution acrylamide gel electrophoresis of histonesArchives of Biochemistry and Biophysics, 1969