Haemolytic micromethod for rapid estimation of toxic alfalfa saponin
Open Access
- 1 January 2015
- journal article
- Published by Polish Botanical Society in Acta Agrobotanica
- Vol. 32 (1) , 5-11
- https://doi.org/10.5586/aa.1979.001
Abstract
On a plate covered with a thin layer of blood suspension with gelatine 10 microliters of alfalfa juice or alfalfa meal extract is spotted. As a result of saponin diffusion and their reaction with blood a haemolytic ring appears, the diameter of which is proportional to the concentration of toxic saponins. Selected by this method low-saponin individual alfalfa plants proved non toxic for fishes and did not inhibit the growth of Trichoderma viride.Keywords
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