Fluorescence immunoassay based on long time correlations of number fluctuations.
- 1 August 1980
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 77 (8) , 4904-4908
- https://doi.org/10.1073/pnas.77.8.4904
Abstract
The development of a fluorescence-based immunoassay technique relying on the physical phenomena of random number fluctuations and diffusion is reported. By determining the autocorrelation of the fluctuations in the fluorescent intensity, this method is able to measure the amount of labeled antigen or antibody that is bound to micrometer-sized carrier particles in solution. The principal advantage of this technique is its insensitivity to small, fast-diffusing sources. It also discriminates against weakly fluorescent contaminants of size comparable to the carrier particles. These attributes were demonstrated by using 2 model systems: a human IgG assay and an idealized system consisting of polystyrene fluorescent spheres and rhodamine dye.This publication has 5 references indexed in Scilit:
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