SEX STEROID BINDING PLASMA PROTEIN (SBP)
- 1 December 1970
- journal article
- Published by Oxford University Press (OUP) in Acta Endocrinologica
- Vol. 65 (1_Suppl) , S204-S224
- https://doi.org/10.1530/acta.0.065s204
Abstract
The detection, preparation from plasma Cohn fraction IV and purification of SBP are reported. The association constant has the value of K = 1.2 and 0.5 109 M−1 at 4°C for testosterone and oestradiol, respectively. A preparation homogeneous by the criteria of polyacrylamide gel electrophoresis, binding studies and ultracentrifugation is obtained; sedimentation equilibrium experiments give a molecular weight of approx. 52 000, confirming the approximative value obtained from Sephadex filtration.Keywords
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