Expression of Klebsiella nif and his Genes in Salmonella typhimurium

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Abstract
Derivatives of S. typhimurium carrying F'' or P'' plasmids with Klebsiella nif and his genes had specific nitrogenase activities similar to Klebsiella in selective conditions, even to showing hyperinduction under Ar. No evidence was obtained for catabolite repression of normal nif expression, but dibutyl cyclic[c]AMP often augmented hyperinduction. In non-selective conditions the Klebsiella his nif determinants were rapidly lost from the plasmids; the low levels of nif expression and temperature-sensitive his expression previously reported were probably due to ready loss of his nif in the test conditions used.