Polyelectrolyte precipitation of proteins: I. The effect of reactor conditions
- 5 September 1988
- journal article
- research article
- Published by Wiley in Biotechnology & Bioengineering
- Vol. 32 (6) , 777-785
- https://doi.org/10.1002/bit.260320609
Abstract
Lysozyme was recovered from egg white by continuous precipitation with polyacrylic acid (molecular weight of 4 × 106). Precipitator residence time and shear rate had significant effects on the size distribution of the precipitate, but no clear effects on the compositions. Precipitate mean size increased with higher shear, indicating growth phenomena predominating over breakage. Also, an enhancement of growth rate at small sizes was noted. The Camp number successfully characterized the interaction of shear rate and residence time on the particle size.This publication has 21 references indexed in Scilit:
- The formation and growth of protein precipitates in a continuous stirred‐tank reactorAIChE Journal, 1986
- Primary particle formation in protein precipitationBiotechnology & Bioengineering, 1985
- Adsorption and desorption of flexible polymer chains in flowing systemsJournal of Colloid and Interface Science, 1985
- Particle size reduction by breakage in biological wastewater treatmentBiotechnology & Bioengineering, 1983
- Shear disruption of soya protein precipitate particles and the effect of aging in a stirred tankBiotechnology & Bioengineering, 1982
- PROTEIN PRECIPITATION-ANALYSIS OF PARTICLE SIZE DISTRIBUTION AND KINETICSChemical Engineering Communications, 1981
- Mechanisms of Deaggregation for Clay-Polymer Flocs in Turbulent SystemsIndustrial & Engineering Chemistry Fundamentals, 1980
- Effect of Shear on Polymer Aided Flocculation of SuspensionsIndustrial & Engineering Chemistry Product Research and Development, 1978
- Flocculation of Neutralized Mine Drainage With PolymersWater Quality Research Journal, 1976
- Flocculation and Flocculation BasinsTransactions of the American Society of Civil Engineers, 1955