High-performance liquid chromatography using spherical aggregates of hydroxyapatite micro-crystals as adsorbent

Open Access
Publisher Website
Google Scholar
Abstract
High‐performance liquid chromatography (HPLC) using spherical aggregates of hydroxyapatite (HA) microcrystals as adsorbent has been developed; preliminary performance tests were carried out by using several types of protein. In comparison with previously developed plate‐like HA packed columns for HPLC, spherical HA packed columns show considerably high chromatographic resolutions in spite of extremely reduced column lengths of 0.5–3 cm. The pressure generated by the latter columns is much higher than that generated by the former, however.