A normalised scale for structural genomics target ranking: The OB‐Score

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Abstract
Target selection and ranking is fundamental to structural genomics. We present a Z-score scale, the "OB-Score", to rank potential targets by their predicted propensity to produce diffraction-quality crystals. The OB-Score is derived from a matrix of predicted isoelectric point and hydroppobicity values for nonredundant PDB entries solved to <= 3.0 angstrom against a background of UniRef50. A highly significant difference was found between the OB-Scores for TargetDB test datasets. A wide range of OR-Scores was observed across 241 proteomes and within 7868 PfamA families; 73.4% of PfamA families contain >= 1 member with a high OB-Score, presenting favourable candidates for structural studies. (c) 2006 Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies.