Synthesis and coding properties of dinucleoside diphosphates containing alkyl pyrimidines which are formed by the action of carcinogens on nucleic acids
- 1 January 1979
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 6 (4) , 1709-1719
- https://doi.org/10.1093/nar/6.4.1709
Abstract
Dinucleoside diphosphates of the general type pGpN have been prepared enzymatically using ribonuclease N1. Alkylated uridines or cytidines, which are products of carcinogens acting on nucleic acids, were tested in dinucleoside diphosphates for their ability to stimulate the binding of Ala- or Val-tRNA to ribosomes. O2-Ethyl C and 3-methyl C functioned as U, but not as C. In contrast, 3-methyl U behaved as C, but not as U. Both O2 and O4-ethyl U could be recognized as C or U, although binding in both cases was weak. Thus, modifications of the hydrogen-bonding sites of U or C causes miscoding and could be considered to represent mutagenic reactions.Keywords
This publication has 21 references indexed in Scilit:
- O2- and O4-Alkyl pyrimidine nucleosides: stability of the glycosyl bond and of the alkyl group as a function of pHBiochemistry, 1978
- "Two out of three": an alternative method for codon reading.Proceedings of the National Academy of Sciences, 1978
- Preparation and template activities of polynucleotides containing O2- and O4-alkyluridine.Proceedings of the National Academy of Sciences, 1978
- All oxygens in nucleic acids react with carcinogenic ethylating agentsNature, 1976
- Reaction of diazoalkanes with 1-substituted 2, 4-dioxopyrimidines. Formation of O2, N-3 and O4-alkyl products.Nucleic Acids Research, 1976
- The chemical effects of nucleic acid alkylation and their relation to mutagenesis and carcinogenesis.1975
- Preparative enzymic synthesis of nucleoside-5'-phosphates.1975
- RNA Codewords and Protein SynthesisScience, 1964
- The dependence of cell-free protein synthesis in E. coli upon naturally occurring or synthetic polyribonucleotidesProceedings of the National Academy of Sciences, 1961
- PREPARATION AND PROPERTIES OF HIGH MOLECULAR WEIGHT POLYMERS OF N-METHYLURIDYLIC ACID1961