Giemsa-banding pattern of chromosomes in Phaseolus vulgaris L.

1 January 1978

journal article
research article
Published by International Society of Cytology in CYTOLOGIA

Vol. 43 (3/4) , 581-588
https://doi.org/10.1508/cytologia.43.581

Abstract

The Giemsa-banding pattern of diploid and polytene chromosomes following the BSG [barium hydroxide-saline-Giemsa] technique and trypsin treatment in P. vulgaris cv. Tsanava Red 41 (2n = 22) is reported. All chromosomes except 1 metacentric pair show either terminal or intercalary or both types of bands. One of the metacentric pairs shows centromeric band. One of the chromosomes with secondary constriction is heteromorphic. The percentage of the Giesma-banding over the diploid complement was 70%. This Giemsa-banding technique is suitable for detection of the chromosomes in the karyotype.

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