Primary structure of the replication initiation protein of plasmid R6K.

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Abstract
The cistron of the replication initiation protein of plasmid R6K was cloned into the single-strand DNA vectors M13mp8 and M13mp9 and its complete nucleotide sequence was determined. The amino acid sequence of the initiator protein as predicted from its nucleotide sequence shows that the protein is lysine rich and weakly basic and has a MW of 35,000, which is in close agreement with that estimated from the mobility in sodium dodecyl sulfate/acrylamide gels. The secondary structure of the protein, approximated by the probabilistic methods of Chou and Fasman, suggests an NH2-terminal domain of primarily positively charged .alpha.-helical structure, a core region of interspersed short stretches of random coils and .beta.-sheets and -turns, and a COOH-terminal domain of .alpha.-helix.