Optimal Protocol for Processing High volume and Peds Plus™Blood Cultures by the BACTEC NR860™
Open Access
- 1 February 1994
- journal article
- research article
- Published by Oxford University Press (OUP) in American Journal of Clinical Pathology
- Vol. 101 (2) , 162-165
- https://doi.org/10.1093/ajcp/101.2.162
Abstract
The optimal incubation period for blood cultures collected in highvolume (Plus 26) and anaerobic (NR7A) media or Peds Plus™ medium and processed by the BACTEC NR860™ was evaluated during a 12- month period. Organisms were recovered from 9% (1528 of 16,870) of blood culture vials (14% [1302 of 9118] of sets). Ninety-eight percent of positive vials were detected by day 5. Thirty-three vials (Plus 26 or NR7A) became positive on day 6 or 7; all positive Peds Plus vials were detected by day 5. Therapy did not change in response to detection of the organisms (26 “occasional” pathogens, 9 “usual” pathogens) in these vials. One usual pathogen had potential clinical significance, but the patient from whom the culture was collected died before it became positive. In the author’s institution, the 5-day protocol for processing blood cultures could be adopted without compromising patient care.Keywords
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