PARTIAL SYNCHRONIZATION OF L1210 CELLS BY 5-FLUOROURACIL AND ITS USE IN DRUG COMBINATIONS

  • 1 January 1977
    • journal article
    • research article
    • Vol. 37  (9) , 3204-3208
Abstract
When L1210 [mouse leukemia] cells growing logarithmically were exposed for 8 h to a nonlethal dose of 5-fluorouracil (FU) (0.25 .mu.g/ml), the percentage of cells in the S phase increased from 74.9% in the asynchronous culture to 93% in the Fu treated culture. This resulted in increased cell kill by S-phase specific inhibitors [1-.beta.-D-arabinofuranosylcytosine (ara-C), 5-hydroxy-2-formylpyridinethiosemicarbazone] when they were added to a culture partially synchronized by pretreatment with FU. A 2 h exposure to ara-C alone or ara-C plus FU (added simultaneously to asynchronous culture) gave 28.8 and 25.8% survival, respectively, compared to 6.8% survival when ara-C was added for 2 h to the partially synchronized culture. Eight to 12 h after FU removal, the culture became asynchronous, such that ara-C addition at this time did not result in increased cell kill. Cultures pretreated with FU were also highly sensitive to vincristine and adriamycin. Adriamycin acted synergistically with FU (after 8 h pretreatment) in killing L1210 cells.

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