Detection of numerical chromosome aberrations in brain tumours by fluorescence in situ hybridization on smear preparations of small tumour biopsies
- 1 October 1994
- journal article
- Published by Wiley in Neuropathology and Applied Neurobiology
- Vol. 20 (5) , 432-438
- https://doi.org/10.1111/j.1365-2990.1994.tb00993.x
Abstract
We present a technique for the preparation and interphase cytogenetic analysis of native tumour cell nuclei from smear preparations of small biopsies of human brain tumours. The presence of tumour-specific numerical chromosome aberrations can be determined by fluorescence in situ hybridization (FISH) analysis using chromosome-specific repetitive DNA probes. The FISH analysis of smear preparations provides cytogenetic information about brain tumour samples, within 2 days, avoiding time-consuming and artefact-prone tumour cell culture.Keywords
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